| Carolinas | Fox Soccer Academy Carolinas | Lowcountry United | Lowcountry United | Charlotte Independence Soccer Club | Fox Soccer Academy Carolinas | Charlotte Eagles |
| Desert | Celtic Storm | FC Tucson Youth Soccer Club | Players SC | Downtown Las Vegas Soccer Club | Sparta United Soccer Club | Sparta United Soccer Club |
| Garden State | AB7 Future Academy | Cedar Stars Academy Newark | — | — | — | — |
| Great Lakes North | Michigan Bucks Academy | Michigan Tigers FC | — | — | Michigan Bucks Academy | Michigan Bucks Academy |
| Great Lakes South | St. Louis Scott Gallagher | Cincinnati United Soccer Club | — | — | — | — |
| Heartland | St. Louis Development Academy | Sporting Wichita | Omaha Evolution Soccer Club | Omaha Evolution Soccer Club | Sting Nebraska | Omaha Evolution Soccer Club |
| Mid-Atlantic | Reading Rage Surf | Bethesda SC | — | — | — | — |
| Mountain | ALBION SC Denver | Colorado United SC | — | — | — | — |
| New England | FC Greater Boston Bolts | Intercontinental Football Academy of New England | — | — | — | — |
| North | Elmbrook United | Elmbrook United | Minneapolis United SC | Minneapolis United SC | Elmbrook United | Lakeville SC |
| Northeast | Atlantic United | Atlantic United | — | — | — | — |
| Northern California Coast | Almaden FC | Burlingame Soccer Club | Burlingame Soccer Club | Elk Grove Soccer | Almaden FC | Elk Grove Soccer |
| Northern California Redwood | Roseville Soccer Club | Lamorinda Soccer Club | Lamorinda Soccer Club | Eastshore Alliance Fútbol Club | ALBION SC Merced | ALBION SC Merced |
| Pacific Northwest | Washington East Surf SC | Western Washington Surf SC | Sozo FC | Atletico Azul | Seattle Celtic | Seattle Celtic |
| Pioneer | Dallas Surf | Dallas Hornets East | Dallas Surf | 210 FC | Ozark United FC Academy | Aspire FC |
| South | ALBION SC Atlanta | Inter Atlanta FC | Inter Atlanta FC | Covered Bridge | Roswell Soccer Club | Southern Soccer Academy |
| Southern California | Strikers FC | Laguna United FC | Laguna United FC | Laguna United FC | Strikers FC | ALBION SC San Diego |
| Sunshine North | United Soccer Alliance | Orlando City Lake Nona | Orlando City Lake Nona | Orlando City Lake Nona | DME Academy Sarasota | NONA Soccer Academy |
| Sunshine South | Key Biscayne SC | Key Biscayne SC | Weston FC | Azzurri Storm Soccer Club | Key Biscayne SC | Weston FC |
| Virginia | Springfield SYC | Loudoun Soccer Club | Virginia Revolution SC | The St. James | Virginia Rush | Northern Virginia United |